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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHOP; GADD153 Monoclonal antibody, PBS Only

CHOP; GADD153 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

4F3G1

Cat no : 66741-1-PBS

Synonyms

C/EBP homologous protein, C/EBP homologous protein 10, CEBPZ, CHOP, CHOP 10, CHOP; GADD153, CHOP10, DDIT 3, DDIT3, GADD153

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHOP; GADD153 Monoclonal antibody (66741-1-Ig)

    WB analysis using 66741-1-Ig (same clone as 66741-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using CHOP; GADD153 Monoclonal antibody (66741-1-Ig)

    WB analysis using 66741-1-Ig (same clone as 66741-1-PBS)

    Un-treated and Tunicamycin treated HepG2 lysates were subjected to SDS PAGE followed by western blot with 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CHOP; GADD153 Monoclonal antibody (66741-1-Ig)

    IHC staining of human cervical cancer using 66741-1-Ig (same clone as 66741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using CHOP; GADD153 Monoclonal antibody (66741-1-Ig)

    IHC staining of human cervical cancer using 66741-1-Ig (same clone as 66741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CHOP; GADD153 Monoclonal antibody (66741-1-Ig)

    IHC staining of human skin cancer using 66741-1-Ig (same clone as 66741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using CHOP; GADD153 Monoclonal antibody (66741-1-Ig)

    IHC staining of human skin cancer using 66741-1-Ig (same clone as 66741-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 66741-1-Ig (CHOP; GADD153 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66741-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66741-1-PBS targets CHOP; GADD153 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CHOP; GADD153 fusion protein Ag7354 相同性解析による交差性が予測される生物種
Full Name DNA-damage-inducible transcript 3
Calculated molecular weight 19 kDa
Observed molecular weight 30 kDa
GenBank accession numberBC003637
Gene symbol DDIT3
Gene ID (NCBI) 1649
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CHOP, also known as GADD153 or DDIT3, is a highly conserved gene in both the structural and regulatory regions. Imposed by unfolded and misfolded proteins, CHOP is significantly induced by ER stress. CHOP is considered a proapoptotic marker of ER stress dependent cell death. CHOP acts as a dominant-negative inhibitor of the transcription factor C/EBP and LAP. It may play an important role in the malignant transformation of nevus to melanoma. The calculated molecular weight of CHOP is 19 kDa, but the protein migrates on an SDS-PAGE gel with an observed molecular mass of 29 kDa (PMID: 1547942).