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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHORDC1 Polyclonal antibody

CHORDC1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25525-1-AP

Synonyms

CHP-1, CHP1, CHP 1, CHORD-containing protein 1, CHORD domain-containing protein 1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHORDC1 Polyclonal antibody (25525-1-AP)

    WB analysis using 25525-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25525-1-AP (CHORDC1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CHORDC1 Polyclonal antibody (25525-1-AP)

    IF Staining of HEK-293 using 25525-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HEK-293 cells using CHORDC1 antibody (25525-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells, K-562 cells
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25525-1-AP targets CHORDC1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHORDC1 fusion protein Ag18315 相同性解析による交差性が予測される生物種
Full Name cysteine and histidine-rich domain (CHORD)-containing 1
Calculated molecular weight 332 aa, 37 kDa
Observed molecular weight37 kDa
GenBank accession numberBC072461
Gene symbol CHORDC1
Gene ID (NCBI) 26973
RRIDAB_2918091
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHORDC1 antibody 25525-1-APDownload protocol
IF protocol for CHORDC1 antibody 25525-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols