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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CHRNA7/CHRFAM7A Polyclonal antibody (21586-1-AP)

    WB analysis of Jurkat using 21586-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21586-1-AP (CHRNA7/CHRFAM7A antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CHRNA7/CHRFAM7A Polyclonal antibody (21586-1-AP)

    WB analysis of Jurkat using 21586-1-AP

    WB result of CHRNA7/CHRFAM7A antibody (21586-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7/CHRFAM7A transfected Jurkat cells.

  • Western Blot (WB) analysis of Jurkat cells using CHRNA7/CHRFAM7A Polyclonal antibody (21586-1-AP)

    WB analysis of Jurkat using 21586-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 21586-1-AP (CHRNA7/CHRFAM7A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  • Featured Product
  • KD/KO Validated

CHRNA7/CHRFAM7A Polyclonal antibody

CHRNA7/CHRFAM7A Polyclonal Antibody for WB, ELISA
Cat No. 21586-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CHRFAM7A, CHRNA7, CHRNA7 DR1, CHRNA7 FAM7A fusion protein, CHRNA7/CHRFAM7A, D 10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21586-1-AP targets CHRNA7/CHRFAM7A in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNA7/CHRFAM7A fusion protein Ag16151 相同性解析による交差性が予測される生物種
Full Name CHRNA7 (cholinergic receptor, nicotinic, alpha 7, exons 5-10) and FAM7A (family with sequence similarity 7A, exons A-E) fusion
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC101348
Gene Symbol CHRNA7/CHRFAM7A
Gene ID (NCBI) 89832
RRIDAB_10734326
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ494W8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Neuronal acetylcholine receptor subunit alpha-7, encoded by the CHRNA7 gene, is involved in cognition through interneuron modulation of 4-(2-aminoethyl)benzene-1,2-diol and glutamate signaling. CHRNA7 gene is genetically linked to multiple disorders with cognitive deficits. A partial duplication of CHRNA7 (CHRFAM7A) is found in humans. CHRFAM7A gene encodes the CHRNA7-FAM7A fusion protein. Expression of CHRFAM7A has been reported in brain, peripheral blood lymphocytes (PBLs), and a broad range of epithelial cells (PMID: 21718690; 23553139; 25681457). CHRFAM7A has been associated with many neuropsychiatric disorders (PMID: 25906356; 25701707). This antibody, raised against 241-386 amino acids of human CHRFAM7A protein, recognizes both CHRNA7 and CHRFAM7A.

Protocols

Product Specific Protocols
WB protocol for CHRNA7/CHRFAM7A antibody 21586-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols