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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHRM3 Polyclonal antibody (21978-1-AP)

    WB analysis using 21978-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21978-1-AP (CHRM3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CHRM3 Polyclonal antibody

CHRM3 Polyclonal Antibody for WB, ELISA
Cat No. 21978-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

HM3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21978-1-AP targets CHRM3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRM3 fusion protein Ag17791 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, muscarinic 3
Calculated molecular weight 590 aa, 66 kDa
Observed molecular weight60-66 kDa
GenBank accession numberBC121026
Gene Symbol CHRM3
Gene ID (NCBI) 1131
RRIDAB_3085681
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20309
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CHRM3 (M3 muscarinic acetylcholine receptor) is a major player in many kinds of cancer (PMID: 26175851). The expression of muscarinic receptors, mainly the CHRM3, is amplified in several types of tumors, including colon cancer, prostate cancer, endometrial carcinoma, and gastric cancer. Colon cancer overexpresses CHRM3, and post-M3R signaling promotes cell growth via the activation of the epidermal growth factor receptors/ERK and protein kinase C/p38 mitogen-activated protein kinase (MAPK) signaling pathways (PMID: 37744266).

Protocols

Product Specific Protocols
WB protocol for CHRM3 antibody 21978-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols