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Validation Data Gallery

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  • Western Blot (WB) analysis of Raji cells using CHRNA10 Polyclonal antibody (55291-1-AP)

    WB analysis of Raji using 55291-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 55291-1-AP (CHRNA10 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CHRNA10 Polyclonal antibody (55291-1-AP)

    WB analysis of Jurkat using 55291-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55291-1-AP (CHRNA10 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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CHRNA10 Polyclonal antibody

CHRNA10 Polyclonal Antibody for WB, ELISA
Cat No. 55291-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CHRNA10, NACHR alpha 10, NACHRA10

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inRaji cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55291-1-AP targets CHRNA10 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 10
Calculated molecular weight 50 kDa
Observed molecular weight 50-52 kDa
GenBank accession numberNM_020402
Gene Symbol CHRNA10
Gene ID (NCBI) 57053
RRIDAB_10860270
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZZ6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

CHRNA10, also named as NACHRA10, belongs to the ligand-gated ion channel (TC 1.A.9) family, Acetylcholine receptor (TC 1.A.9.1) subfamily and Alpha-10/CHRNA10 sub-subfamily. It is an ionotropic receptor with a probable role in the modulation of auditory stimuli. In the ear, CHRNA10 may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. It may protect against acoustic trauma. This antibody is specific to CHRNA10.

Protocols

Product Specific Protocols
WB protocol for CHRNA10 antibody 55291-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols