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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHRNA3 Polyclonal antibody (10333-1-AP)

    WB analysis using 10333-1-AP (same clone as 10333-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse thymus tissue using CHRNA3 Polyclonal antibody (10333-1-AP)

    WB analysis of mouse thymus using 10333-1-AP (same clone as 10333-1-PBS)

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using CHRNA3 Polyclonal antibody (10333-1-AP)

    WB analysis of HepG2 using 10333-1-AP (same clone as 10333-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using CHRNA3 Polyclonal antibody (10333-1-AP)

    WB analysis of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using CHRNA3 Polyclonal antibody (10333-1-AP)

    WB analysis of HEK-293 using 10333-1-AP (same clone as 10333-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10333-1-AP (CHRNA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHRNA3 Polyclonal antibody (10333-1-AP)

    IHC staining of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHRNA3 Polyclonal antibody (10333-1-AP)

    IHC staining of mouse brain using 10333-1-AP (same clone as 10333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10333-1-AP (CHRNA3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using CHRNA3 Polyclonal antibody (10333-1-AP)

    IHC staining of human prostate cancer using 10333-1-AP (same clone as 10333-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10333-1-AP (CHRNA3 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 10333-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CHRNA3 Polyclonal antibody, PBS Only

CHRNA3 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 10333-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Neuronal acetylcholine receptor subunit alpha-3, NACHRA3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10333-1-PBS targets CHRNA3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNA3 fusion protein Ag0272 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 3
Calculated molecular weight 57 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC000513
Gene Symbol CHRNA3
Gene ID (NCBI) 1136
RRIDAB_2080511
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32297
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

CHRNA3, also named as NACHRA3, LNCR2 and PAOD2, is neuronal type nAChR subunits. It is a member of the nicotinic acetylcholine receptor family, which plays an important role in calcium regulation, neuronal development, and cognitive functions. Polymorphisms in this gene have been associated with an increased risk of smoking initiation and an increased susceptibility to lung cancer.