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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of Jurkat using 13516-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A549 cells using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of A549 using 13516-1-AP

    A549 cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse stomach tissue using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of mouse stomach using 13516-1-AP

    mouse stomach tissue were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse bladder tissue using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of mouse bladder using 13516-1-AP

    mouse bladder tissue were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using CHRNA5 Polyclonal antibody (13516-1-AP)

    WB analysis of A431 using 13516-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 13516-1-AP (CHRNA5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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CHRNA5 Polyclonal antibody

CHRNA5 Polyclonal Antibody for WB, ELISA
Cat No. 13516-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

CHRNA5, LNCR2, NACHRA5

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, A431 cells, A549 cells, mouse bladder tissue, mouse stomach tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13516-1-AP targets CHRNA5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNA5 fusion protein Ag4447 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 5
Calculated molecular weight 468 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC033639
Gene Symbol CHRNA5
Gene ID (NCBI) 1138
RRIDAB_2080670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP30532
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.

Protocols

Product Specific Protocols
WB protocol for CHRNA5 antibody 13516-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols