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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using CHRNA5 Monoclonal antibody (66363-1-Ig)

    WB analysis of mouse skeletal muscle using 66363-1-Ig (same clone as 66363-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig skeletal muscle tissue using CHRNA5 Monoclonal antibody (66363-1-Ig)

    WB analysis of pig skeletal muscle using 66363-1-Ig (same clone as 66363-1-PBS)

    pig skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using CHRNA5 Monoclonal antibody (66363-1-Ig)

    WB analysis of A549 using 66363-1-Ig (same clone as 66363-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of C2C12 cells using CHRNA5 Monoclonal antibody (66363-1-Ig)

    WB analysis of C2C12 using 66363-1-Ig (same clone as 66363-1-PBS)

    C2C12 cell were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using CHRNA5 Monoclonal antibody (66363-1-Ig)

    WB analysis of human skeletal muscle using 66363-1-Ig (same clone as 66363-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bladder tissue using CHRNA5 Monoclonal antibody (66363-1-Ig)

    IHC staining of human bladder using 66363-1-Ig (same clone as 66363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human bladder tissue using CHRNA5 Monoclonal antibody (66363-1-Ig)

    IHC staining of human bladder using 66363-1-Ig (same clone as 66363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human bladder tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using CHRNA5 Monoclonal antibody (66363-1-Ig)

    IHC staining of human stomach using 66363-1-Ig (same clone as 66363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach tissue using CHRNA5 Monoclonal antibody (66363-1-Ig)

    IHC staining of human stomach using 66363-1-Ig (same clone as 66363-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 66363-1-Ig (CHRNA5 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66363-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

CHRNA5 Monoclonal antibody, PBS Only

CHRNA5 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66363-1-PBS
Clone No.3G8C11

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, pig

Applications

WB, IHC, Indirect ELISA

Synonyms

CHRNA5, LNCR2, NACHRA5

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66363-1-PBS targets CHRNA5 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CHRNA5 fusion protein Ag4447 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 5
Calculated molecular weight 468 aa, 53 kDa
Observed molecular weight 53 kDa
GenBank accession numberBC033639
Gene Symbol CHRNA5
Gene ID (NCBI) 1138
RRIDAB_2881743
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30532
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Nicotinic acetylcholine receptors (nAChRs), such as CHRNA5, are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be (hetero)pentamers composed of homologous subunits. Variants in the CHRNA5 gene are associated with nicotine dependence and lung cancer risk.