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CHRNA7 Monoclonal antibody

CHRNA7 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 60220-1-Ig
Clone No.2H1G3

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, ELISA

CHRNA7, CHRNA7 2, NACHRA7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inMCF7 cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

60220-1-Ig targets CHRNA7 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen CHRNA7 fusion protein Ag15980 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 7
Calculated molecular weight 502 aa, 56 kDa
Observed molecular weight 56 kDa
GenBank accession numberBC037571
Gene Symbol CHRNA7
Gene ID (NCBI) 1139
RRIDAB_11043180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP36544
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin.

Protocols

Product Specific Protocols
WB protocol for CHRNA7 antibody 60220-1-IgDownload protocol
IHC protocol for CHRNA7 antibody 60220-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

J Biol Chem

Functional Human α7 Nicotinic Acetylcholine Receptor (nAChR) Generated from E. coli.

Authors - Tommy S Tillman
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