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  • Featured Product
  • KD/KO Validated

CHRNA7 (48 kDa) Recombinant antibody

CHRNA7 (48 kDa) Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2A10

Cat no : 82848-3-RR

Synonyms

CHRNA7, Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 2A10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHRNA7 Recombinant antibody (82848-3-RR)

    WB analysis using 82848-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-3-RR (CHRNA7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CHRNA7 Recombinant antibody (82848-3-RR)

    WB analysis of Jurkat using 82848-3-RR

    WB result of CHRNA7 antibody (82848-3-RR; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHRNA7 transfected Jurkat cells.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHRNA7 Recombinant antibody (82848-3-RR)

    IHC staining of mouse brain using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CHRNA7 Recombinant antibody (82848-3-RR)

    IHC staining of mouse brain using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat dorsal root ganglion tissue using CHRNA7 Recombinant antibody (82848-3-RR)

    IHC staining of rat dorsal root ganglion using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat dorsal root ganglion tissue using CHRNA7 Recombinant antibody (82848-3-RR)

    IHC staining of rat dorsal root ganglion using 82848-3-RR

    Immunohistochemical analysis of paraffin-embedded rat dorsal root ganglion tissue slide using 82848-3-RR (CHRNA7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 82848-3-RR

    Affinity and Kinetic Characterization of 82848-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-3-RR against Human CHRNA7 (48 kDa) were performed. The affinity constant is 2.04 nM.

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Tested Applications

Positive WB detected inrat brain tissue, Jurkat cells
Positive IHC detected inmouse brain tissue, rat dorsal root ganglion tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82848-3-RR targets CHRNA7 (48 kDa) in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHRNA7 (48 kDa) fusion protein Ag15980 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 7
Calculated molecular weight 502 aa, 56 kDa
Observed molecular weight~50 kDa
GenBank accession numberBC037571
Gene symbol CHRNA7
Gene ID (NCBI) 1139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

Protocols

Product Specific Protocols
WB protocol for CHRNA7 (48 kDa) antibody 82848-3-RRDownload protocol
IHC protocol for CHRNA7 (48 kDa) antibody 82848-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols