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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHRNA7 Recombinant antibody

CHRNA7 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240117D12

Cat no : 82848-4-RR

Synonyms

Neuronal acetylcholine receptor subunit alpha-7, NACHRA7, CHRNA7 2, 240117D12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHRNA7 Recombinant antibody (82848-4-RR)

    WB analysis using 82848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CHRNA7 Recombinant antibody (82848-4-RR)

    WB analysis using 82848-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82848-4-RR (CHRNA7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CHRNA7 Recombinant antibody (82848-4-RR)

    FC experiment of SH-SY5Y using 82848-4-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Chrna7 Recombinant Antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of Jurkat cells using CHRNA7 Recombinant antibody (82848-4-RR)

    FC experiment of Jurkat using 82848-4-RR

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug CHRNA7 Recombinant antibody (82848-4-RR, Clone:240117D12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82848-4-RR

    Affinity and Kinetic Characterization of 82848-4-RR

    Biolayer interferometry (BLl) kinetic assays of 82848-4-RR against Human CHRNA7 were performed. The affinity constant is below 1 pM.

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Tested Applications

Positive WB detected inrat brain tissue, mouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells, Jurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 82848-4-RR is CHRNA7 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CHRNA7 fusion protein Ag15980 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, alpha 7
Calculated molecular weight 502 aa, 56 kDa
Observed molecular weight50 kDa, 56 kDa
GenBank accession numberBC037571
Gene symbol CHRNA7
Gene ID (NCBI) 1139
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Nicotinic acetylcholine receptors (nAChRs) are cholinergic receptors that form ligand-gated ion channels that mediate fast signal transmission at synapses. CHRNA7 (neuronal acetylcholine receptor subunit alpha-7) forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. CHRNA7, so that like CHRNA7, the epithelial CHRFAM7A ORF encodes a protein (48 kDa) that differs from the CHRNA7 (58 kDa) by a distinct amino terminus and molecular weight.(PMID: 25473097)

Protocols

Product Specific Protocols
WB protocol for CHRNA7 antibody 82848-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols