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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHRNB2 Polyclonal antibody

CHRNB2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 17844-1-AP

Synonyms

CHRNB2, EFNL3, nAChRB2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of SH-SY5Y cells using CHRNB2 Polyclonal antibody (17844-1-AP)

    WB analysis of SH-SY5Y using 17844-1-AP

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 17844-1-AP (CHRNB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CHRNB2 Polyclonal antibody (17844-1-AP)

    WB analysis of mouse brain using 17844-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17844-1-AP (CHRNB2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human brain tissue using CHRNB2 Polyclonal antibody (17844-1-AP)

    IHC staining of human brain using 17844-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17844-1-AP (CHRNB2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using CHRNB2 Polyclonal antibody (17844-1-AP)

    IHC staining of human brain using 17844-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 17844-1-AP (CHRNB2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSH-SY5Y cells, mouse brain tissue
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

The immunogen of 17844-1-AP is CHRNB2 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHRNB2 fusion protein Ag12208 相同性解析による交差性が予測される生物種
Full Name cholinergic receptor, nicotinic, beta 2 (neuronal)
Calculated molecular weight 502 aa, 57 kDa
Observed molecular weight 60-65 kDa
GenBank accession numberBC075041
Gene symbol CHRNB2
Gene ID (NCBI) 1141
RRIDAB_2878450
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHRNB2 antibody 17844-1-APDownload protocol
IHC protocol for CHRNB2 antibody 17844-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Elife

Hsp47 promotes biogenesis of multi-subunit neuroreceptors in the endoplasmic reticulum

Authors - Ya-Juan Wang
View Article