Featured Product
KD/KO Validated

CHRNB4 Polyclonal antibody

Name: CHRNB4 Polyclonal antibody
Brand: Proteintech
SKU: 22192-1-AP

CHRNB4 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22192-1-AP

Synonyms

Neuronal acetylcholine receptor subunit beta-4, CHRNB 4

Validation Data Gallery

Filter:

AllWBFC

WB analysis of SH-SY5Y using 22192-1-AP
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 22192-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of mouse testis using 22192-1-AP
mouse testis tissue were subjected to SDS PAGE followed by western blot with 22192-1-AP (CHRNB4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
FC experiment of SH-SY5Y using 22192-1-AP
1x10^6 SH-SY5Y cells were surface stained with 0.4 ug CHRNB4 Polyclonal antibody (22192-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

-/ -

Cat No. 22192-1-AP

Size/Concentration

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	SH-SY5Y cells, HeLa cells, mouse testis tissue
Positive FC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 3 publications below
FC	See 1 publications below

Product Information

22192-1-AP targets CHRNB4 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CHRNB4 fusion protein Ag16767 相同性解析による交差性が予測される生物種
Full Name	cholinergic receptor, nicotinic, beta 4
Calculated molecular weight	498 aa, 56 kDa
Observed molecular weight	48-60 kDa
GenBank accession number	BC096080
Gene symbol	CHRNB4
Gene ID (NCBI)	1143
RRID	AB_11232227
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for CHRNB4 antibody 22192-1-AP	Download protocol
FC protocol for CHRNB4 antibody 22192-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	FC	Mar Drugs Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA. Authors - Zhihua Sun View Article
human	WB	Eur J Pharmacol Identification of nicotinic acetylcholine receptor subunits in different lung cancer cell lines and the inhibitory effect of alpha-conotoxin TxID on lung cancer cell growth. Authors - Jiang Qian View Article
human	WB	Cancers (Basel) An Integrated Genomic Strategy to Identify CHRNB4 as a Diagnostic/Prognostic Biomarker for Targeted Therapy in Head and Neck Cancer. Authors - Yi-Hsuan Chuang KO Validated View Article
human	WB	Mar Drugs Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets. Authors - Yiqiao Liu View Article

製品

アプリケーション

cGMPグレード製品

サポート

カスタム対応

プロモーション

企業情報

抗体

免疫アッセイ

サイトカイン・増殖因子

ChromoTek VHH（Nanobody®）試薬

サポートツール・試薬

ゲノミクス試薬

研究分野

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CHRNB4 Polyclonal antibody

CHRNB4 Polyclonal Antibody for WB, ELISA

Validation Data Gallery

WB analysis of SH-SY5Y using 22192-1-AP

WB analysis of HeLa using 22192-1-AP

WB analysis of mouse testis using 22192-1-AP

FC experiment of SH-SY5Y using 22192-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Protocols

Publications

Differential Expression of Nicotine Acetylcholine Receptors Associates with Human Breast Cancer and Mediates Antitumor Activity of αO-Conotoxin GeXIVA.

Identification of nicotinic acetylcholine receptor subunits in different lung cancer cell lines and the inhibitory effect of alpha-conotoxin TxID on lung cancer cell growth.

An Integrated Genomic Strategy to Identify CHRNB4 as a Diagnostic/Prognostic Biomarker for Targeted Therapy in Head and Neck Cancer.

Cervical Cancer Correlates with the Differential Expression of Nicotinic Acetylcholine Receptors and Reveals Therapeutic Targets.