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Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using CHST1 Polyclonal antibody (12504-1-AP)

    WB analysis of Jurkat using 12504-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 12504-1-AP (CHST1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using CHST1 Polyclonal antibody (12504-1-AP)

    WB analysis of mouse brain using 12504-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 12504-1-AP (CHST1 Antibody) at dilution of 1:600 incubated at 4 degree celsius over night.

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CHST1 Polyclonal antibody

CHST1 Polyclonal Antibody for WB, ELISA
Cat No. 12504-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

C6ST, CHST1, GST 1, KS6ST, KSGal6ST, KSST

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12504-1-AP targets CHST1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHST1 fusion protein Ag3183 相同性解析による交差性が予測される生物種
Full Name carbohydrate (keratan sulfate Gal-6) sulfotransferase 1
Calculated molecular weight 411 aa, 47 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC022567
Gene Symbol CHST1
Gene ID (NCBI) 8534
RRIDAB_2877862
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43916
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHST1 antibody 12504-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols