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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CHST14 Polyclonal antibody (17749-1-AP)

    WB analysis of HEK-293 using 17749-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17749-1-AP (CHST14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CHST14 Polyclonal antibody (17749-1-AP)

    WB analysis of HepG2 using 17749-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17749-1-AP (CHST14 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using CHST14 Polyclonal antibody (17749-1-AP)

    IHC staining of human placenta using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using CHST14 Polyclonal antibody (17749-1-AP)

    IHC staining of human placenta using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CHST14 Polyclonal antibody (17749-1-AP)

    IHC staining of human brain using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using CHST14 Polyclonal antibody (17749-1-AP)

    IHC staining of human brain using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CHST14 Polyclonal antibody (17749-1-AP)

    IHC staining of human spleen using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human spleen tissue using CHST14 Polyclonal antibody (17749-1-AP)

    IHC staining of human spleen using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHST14 Polyclonal antibody (17749-1-AP)

    IHC staining of human kidney using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using CHST14 Polyclonal antibody (17749-1-AP)

    IHC staining of human kidney using 17749-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17749-1-AP (CHST14 antibody) at dilution of 1:50 (under 40x lens).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

CHST14 Polyclonal antibody

CHST14 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 17749-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CHST14, D4ST 1, D4ST1, Dermatan 4 sulfotransferase 1, HD4ST, hD4ST1, HNK1ST

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HepG2 cells
Positive IHC detected inhuman placenta tissue, human brain tissue, human kidney tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

17749-1-AP targets CHST14 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHST14 fusion protein Ag12014 相同性解析による交差性が予測される生物種
Full Name carbohydrate (N-acetylgalactosamine 4-0) sulfotransferase 14
Calculated molecular weight 376 aa, 43 kDa
Observed molecular weight 45-50 kDa
GenBank accession numberBC049214
Gene Symbol CHST14
Gene ID (NCBI) 113189
RRIDAB_2079051
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8NCH0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHST14 antibody 17749-1-APDownload protocol
IHC protocol for CHST14 antibody 17749-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Transl Oncol

A comprehensive analysis of the expression, immune infiltration, prognosis and partial experimental validation of CHST family genes in gastric cancer

Authors - Jinbo Zhan
View Article