Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
Product Information
26027-1-AP targets CHST2 in WB, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CHST2 fusion protein Ag23293 相同性解析による交差性が予測される生物種 |
| Full Name | carbohydrate (N-acetylglucosamine-6-O) sulfotransferase 2 |
| Observed molecular weight | 50-52 kDa, 58-60 kDa |
| GenBank accession number | BC105010 |
| Gene Symbol | CHST2 |
| Gene ID (NCBI) | 9435 |
| RRID | AB_2880342 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y4C5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CHST2 encodes N-Acetylglucosamine-6-sulfotransferase-1 (GlcNAc6ST-1), a Golgi-resident glycoprotein that is responsible for sulfation of the L-selectin ligand on endothelial cells. Two isoforms of CHST2 exist due to the alternative splicing. This antibody recognizes both short form and long form, which migrates around 50-52 kDa and 58-60 kDa, respectively.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CHST2 antibody 26027-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Death Dis CHST2-mediated sulfation of MECA79 antigens is critical for breast cancer cell migration and metastasis
|