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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CHTF18 Polyclonal antibody (13030-1-AP)

    WB analysis using 13030-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13030-1-AP (CHTF18 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using CHTF18 Polyclonal antibody (13030-1-AP)

    WB analysis of Y79 using 13030-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 13030-1-AP (CHTF18 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human medulloblastoma tissue using CHTF18 Polyclonal antibody (13030-1-AP)

    IHC staining of human medulloblastoma using 13030-1-AP

    Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 13030-1-AP (CHTF18 antibody) at dilution of 1:100 (under 10x lens).

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CHTF18 Polyclonal antibody

CHTF18 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 13030-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

C16orf41, C321D2.2, C321D2.3, C321D2.4, CHL12, CHTF18, Ctf18, hCTF18, RUVBL

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, Y79 cells
Positive IHC detected inhuman medulloblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13030-1-AP targets CHTF18 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CHTF18 fusion protein Ag3672 相同性解析による交差性が予測される生物種
Full Name CTF18, chromosome transmission fidelity factor 18 homolog (S. cerevisiae)
Calculated molecular weight 1184 aa, 129 kDa
Observed molecular weight 110 kDa
GenBank accession numberBC018184
Gene Symbol CHTF18
Gene ID (NCBI) 63922
RRIDAB_2260469
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8WVB6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CHTF18 antibody 13030-1-APDownload protocol
IHC protocol for CHTF18 antibody 13030-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols