Validation Data Gallery
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Tested Applications
| Positive WB detected in | A549 cells, HEK-293 cells, U2OS cells |
| Positive IHC detected in | human lung cancer tissue, human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 1 publications below |
Product Information
23199-1-AP targets CIP2A in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CIP2A fusion protein Ag19598 相同性解析による交差性が予測される生物種 |
| Full Name | KIAA1524 |
| Calculated molecular weight | 905 aa, 102 kDa |
| Observed molecular weight | 80-102 kDa |
| GenBank accession number | BC130564 |
| Gene Symbol | CIP2A |
| Gene ID (NCBI) | 57650 |
| RRID | AB_2918079 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q8TCG1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Cancerous inhibitor of protein phosphatase 2A (CIP2A) was originally identified as a cellular protein phosphatase 2A (PP2A) inhibitor, and has been shown to control oncogenic cellular signals by suppressing the tumor suppressor PP2A. CIP2A overexpression has been found in several human malignancies including breast cancer, hepatocellular carcinoma, gastric cancer, head and neck cancer, colon cancer, prostate cancer and non-small cell lung cancer. CIP2A is a 83~102 kDa cytoplasmic protein. ( PMID: 26824320, PMID: 25458953, PMID: 29720672)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CIP2A antibody 23199-1-AP | Download protocol |
| IHC protocol for CIP2A antibody 23199-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biochim Biophys Acta Mol Basis Dis Loss of function variant in CIP2A associated with female infertility with early embryonic arrest and fragmentation | ||
Smart Med TP53 mutation-related senescence is an indicator of hepatocellular carcinoma patient outcomes from multiomics profiles |