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  • Featured Product
  • KD/KO Validated

CIRBP Polyclonal antibody

CIRBP Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 10209-2-AP

Synonyms

CIRP, A18HNRNP, A18 hnRNP

Validation Data Gallery

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  • Western Blot (WB) analysis of A549 cells using CIRBP Polyclonal antibody (10209-2-AP)

    WB analysis of A549 cells using 10209-2-AP

    WB result of CIRBP antibody (10209-2-AP, 1:500) with si-control and si-CIRBP transfected A549 cell.

  • Western Blot (WB) analysis of PC-12 cells using CIRBP Polyclonal antibody (10209-2-AP)

    WB analysis of PC-12 using 10209-2-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CIRBP Polyclonal antibody (10209-2-AP)

    WB analysis using 10209-2-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-13 cells using CIRBP Polyclonal antibody (10209-2-AP)

    WB analysis of PC-13 using 10209-2-AP

    PC-13 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using CIRBP Polyclonal antibody (10209-2-AP)

    WB analysis of HepG2 using 10209-2-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using CIRBP Polyclonal antibody (10209-2-AP)

    WB analysis of HEK-293 using 10209-2-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10209-2-AP (CIRBP antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using CIRBP Polyclonal antibody (10209-2-AP)

    IP experiment of HepG2 using 10209-2-AP

    IP result of anti-CIRBP (IP:10209-2-AP, 3ug; Detection:10209-2-AP 1:500) with HepG2 cells lysate 6000ug.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CIRBP Polyclonal antibody (10209-2-AP)

    IHC staining of human pancreas cancer using 10209-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CIRBP Polyclonal antibody (10209-2-AP)

    IHC staining of human pancreas cancer using 10209-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CIRBP Polyclonal antibody (10209-2-AP)

    IHC staining of mouse pancreas using 10209-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using CIRBP Polyclonal antibody (10209-2-AP)

    IHC staining of mouse pancreas using 10209-2-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CIRBP Polyclonal antibody (10209-2-AP)

    IHC staining of human breast cancer using 10209-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CIRBP Polyclonal antibody (10209-2-AP)

    IHC staining of human breast cancer using 10209-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using CIRBP Polyclonal antibody (10209-2-AP)

    IHC staining of human breast cancer using 10209-2-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10209-2-AP (CIRBP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CIRBP Polyclonal antibody (10209-2-AP)

    IHC staining of human pancreas cancer using 10209-2-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 10209-2-AP (CIRBP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CIRBP Polyclonal antibody (10209-2-AP)

    IF Staining of HEK-293 using 10209-2-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CIRBP antibody (10209-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Flow cytometry (FC) experiment of A549 cells using CIRBP Polyclonal antibody (10209-2-AP)

    FC experiment of A549 using 10209-2-AP

    1X10^6 A549 cells were intracellularly stained with 0.4 ug Anti-Human CIRBP (10209-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inY79 cells, A549 cells, HEK-293 cells, HepG2 cells, PC-12 cells, PC-13 cells, mouse testis tissue, rat testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman pancreas cancer tissue, human breast cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10209-2-AP targets CIRBP in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, squirrel
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CIRBP fusion protein Ag0280 相同性解析による交差性が予測される生物種
Full Name cold inducible RNA binding protein
Calculated molecular weight 172 aa, 19 kDa
Observed molecular weight 19 kDa
GenBank accession numberBC000403
Gene symbol CIRBP
Gene ID (NCBI) 1153
RRIDAB_2080263
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CIRBP, also named as A18HNRNP and CIRP, is a cold-inducible mRNA binding protein that plays a protective role in the genotoxic stress response by stabilizing transcripts of genes involved in cell survival[PMID: 19158277]. CIRBP is also involved in cap-independent translation upon moderate cold-shock. It acts as a translational activator. CIRBP is a suppressive rather stimulatory effect on proliferation[PMID: 19777567]. CIRBP can translocate from nucleus to cytoplasm under stresses such as UV irradation or heat shock. Suppression of CIRBP increased the apoptotic cell population of neural stem cells at moderate low temperature[PMID: 20735994]. This antibody (Catalog# 10209-2-AP) is a rabbit polyclonal antibody raised against the full-length CIRBP of human origin.

Protocols

Product Specific Protocols
WB protocol for CIRBP antibody 10209-2-APDownload protocol
IHC protocol for CIRBP antibody 10209-2-APDownload protocol
IF protocol for CIRBP antibody 10209-2-APDownload protocol
IP protocol for CIRBP antibody 10209-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

RBM3 mediates structural plasticity and protective effects of cooling in neurodegeneration.

Authors - Diego Peretti
View Article
human,squirrelWB

Cell

iPSCs from a Hibernator Provide a Platform for Studying Cold Adaptation and Its Potential Medical Applications.

Authors - Jingxing Ou
View Article
ratWB

Nat Med

Cold-inducible RNA-binding protein (CIRP) triggers inflammatory responses in hemorrhagic shock and sepsis.

Authors - Qiang Xiaoling X
View Article
humanWB

Mol Cell

Altered m6A Modification of Specific Cellular Transcripts Affects Flaviviridae Infection.

Authors - Nandan S Gokhale
View Article
humanWB

Nucleic Acids Res

Alternative splicing induced by bacterial pore-forming toxins sharpens CIRBP-mediated cell response to Listeria infection

Authors - Morgane Corre
View Article
humanWB,IHC

J Exp Clin Cancer Res

Hypoxia-enhanced YAP1-EIF4A3 interaction drives circ_0007386 circularization by competing with CRIM1 pre-mRNA linear splicing and promotes non-small cell lung cancer progression

Authors - Lixia Li
  • KD Validated
View Article