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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CISD1 Monoclonal antibody, PBS Only

CISD1 Monoclonal Antibody for WB, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, chicken, rabbit, pig

Applications

WB, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D7E3

Cat no : 68030-1-PBS

Synonyms

C10orf70, CDGSH iron sulfur domain 1, CISD1, MDS029, mitoNEET, ZCD1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CISD1 Monoclonal antibody (68030-1-Ig)

    WB analysis using 68030-1-Ig (same clone as 68030-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68030-1-Ig (CISD1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CISD1 Monoclonal antibody (68030-1-Ig)

    IHC staining of human pancreas cancer using 68030-1-Ig (same clone as 68030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68030-1-Ig (CISD1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using CISD1 Monoclonal antibody (68030-1-Ig)

    IHC staining of human pancreas cancer using 68030-1-Ig (same clone as 68030-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68030-1-Ig (CISD1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of H9C2 cells using CISD1 Monoclonal antibody (68030-1-Ig)

    IF Staining of H9C2 using 68030-1-Ig (same clone as 68030-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CISD1 antibody (68030-1-Ig, Clone: 1D7E3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68030-1-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68030-1-PBS targets CISD1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, chicken, rabbit, pig samples.

Tested Reactivity Human, mouse, rat, chicken, rabbit, pig
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CISD1 fusion protein Ag8560 相同性解析による交差性が予測される生物種
Full Name CDGSH iron sulfur domain 1
Calculated molecular weight 108 aa, 12 kDa
Observed molecular weight 14-17 kDa
GenBank accession numberBC007043
Gene symbol CISD1
Gene ID (NCBI) 55847
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

MitoNEET, also named CISD1, belongs to a previously uncharacterized ancient family of proteins for which the hallmark is the presence of a unique 39 amino acid CDGSH domain. It is a single-pass type III membrane protein, located in mitochondrion outer membrane and may play a role in regulating maximal capacity for electron transport and oxidative phosphorylation. MitoNEET is a recently identified drug target for a commonly prescribed diabetes drug, Pioglitazone. This antibody recognizing MitoNEET (calculated 12 kDa) as a 17 kDa protein may be due to its posttranslational modification or metal binding activity.