• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CISD2 Recombinant antibody, PBS Only

CISD2 Recombinant Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6H13

Cat no : 82802-10-PBS

Synonyms

CDGSH iron sulfur domain 2, CDGSH2, CISD2, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using CISD2 Recombinant antibody (82802-10-RR)

    WB analysis using 82802-10-RR (same clone as 82802-10-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82802-10-RR (CISD2 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CISD2 Recombinant antibody (82802-10-RR)

    IHC staining of mouse kidney using 82802-10-RR (same clone as 82802-10-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CISD2 Recombinant antibody (82802-10-RR)

    IHC staining of mouse kidney using 82802-10-RR (same clone as 82802-10-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82802-10-RR (CISD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82802-10-PBS in a different storage buffer formulation.

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82802-10-PBS targets CISD2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CISD2 fusion protein Ag4172 相同性解析による交差性が予測される生物種
Full Name CDGSH iron sulfur domain 2
Calculated molecular weight 135 aa, 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC032300
Gene symbol CISD2
Gene ID (NCBI) 493856
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.