CIT Polyclonal antibody
CIT Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
Cat no : 20033-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | A431 cells, HeLa cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 4 publications below |
Product Information
20033-1-AP targets CIT in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | citron (rho-interacting, serine/threonine kinase 21) |
| Calculated molecular weight | 231 kDa |
| Observed molecular weight | 230 kDa, 177 kDa |
| GenBank accession number | NM_007174 |
| Gene symbol | CIT |
| Gene ID (NCBI) | 11113 |
| RRID | AB_10665814 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoforms of CIT.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CIT antibody 20033-1-AP | Download protocol |
| IHC protocol for CIT antibody 20033-1-AP | Download protocol |
| IF protocol for CIT antibody 20033-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Biol E2F-2 Promotes Nuclear Condensation and Enucleation of Terminally Differentiated Erythroblasts. | ||
Toxicol Appl Pharmacol Zinc finger protein 367 exerts a cancer-promoting role in small cell lung cancer by influencing the CIT/LATS2/YAP signaling cascade | ||
Sci Rep A three-gene signature reveals changes in the tumor immune microenvironment in the progression from NAFLD to HCC |
