Validation Data Gallery
Tested Applications
| Positive WB detected in | SH-SY5Y cells, mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, mouse ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | SH-SY5Y cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
15021-1-AP targets CITED2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CITED2 fusion protein Ag7078 相同性解析による交差性が予測される生物種 |
| Full Name | Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2 |
| Calculated molecular weight | 28 kDa |
| Observed molecular weight | 36 kDa, 27 kDa |
| GenBank accession number | BC004377 |
| Gene Symbol | CITED2 |
| Gene ID (NCBI) | 10370 |
| RRID | AB_2080545 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99967 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CITED2, also named as P35srj, is a cAMP-responsive element-binding protein (CBP)/p300-transactivator, with an ED(Glu/Asp)-rich C-terminal domain that acts as an important modulator in the development of the heart [PMID:10552932]. There are two isoforms of CITED2 protein and the calculated molecular weight of them are 24kDa and 28 kDa. The 28 kDa isoform having modification site may migrate to 35 kDa and 24 kDa isoform having modification site may migrate to 27 kDa (PMID: 23082118). It is proposed to be a negative regulator of HIF-1α through its competitive binding to the CH1 domain of CBP/p300, which mediated signaling and to a decrease in HIF-1-responsive genes such as VEGF [PMID:9887100]. In addition, it is also a co-activator of the transcriptional activity of the TFAP2 isoform, which is an extensively expressed nuclear protein with functions in embryogenesis, inflammation, and stress responses[PMID:22735262].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CITED2 antibody 15021-1-AP | Download protocol |
| IHC protocol for CITED2 antibody 15021-1-AP | Download protocol |
| IF protocol for CITED2 antibody 15021-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|