Validation Data Gallery
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Tested Applications
| Positive WB detected in | HepG2 cells, K-562 cells, Jurkat cells |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 1 publications below |
Product Information
17143-1-AP targets CKAP2L in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CKAP2L fusion protein Ag10909 相同性解析による交差性が予測される生物種 |
| Full Name | cytoskeleton associated protein 2-like |
| Calculated molecular weight | 745 aa, 84 kDa |
| Observed molecular weight | 84 kDa |
| GenBank accession number | BC036217 |
| Gene Symbol | CKAP2L |
| Gene ID (NCBI) | 150468 |
| RRID | AB_2080720 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IYA6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CKAP2L antibody 17143-1-AP | Download protocol |
| IHC protocol for CKAP2L antibody 17143-1-AP | Download protocol |
| IP protocol for CKAP2L antibody 17143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Clin Exp Pathol Oncogenic and prognostic role of CKAP2L in hepatocellular carcinoma.
| ||
FASEB J IGF2BP2 promotes ovarian cancer growth and metastasis by upregulating CKAP2L protein expression in an m6 A-dependent manner |