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  • Featured Product
  • KD/KO Validated

CKB Monoclonal antibody, PBS Only

CKB Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B7C3

Cat no : 66764-1-PBS

Synonyms

B CK, CKB, CKBB, Creatine kinase B chain, Creatine kinase B type, creatine kinase, brain

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using CKB Monoclonal antibody (66764-1-Ig)

    WB analysis of HEK-293 using 66764-1-Ig (same clone as 66764-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CKB Monoclonal antibody (66764-1-Ig)

    WB analysis of HeLa using 66764-1-Ig (same clone as 66764-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CKB Monoclonal antibody (66764-1-Ig)

    IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using CKB Monoclonal antibody (66764-1-Ig)

    IHC staining of mouse brain using 66764-1-Ig (same clone as 66764-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66764-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66764-1-PBS targets CKB in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen CKB fusion protein Ag7933 相同性解析による交差性が予測される生物種
Full Name creatine kinase, brain
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC001190
Gene symbol CKB
Gene ID (NCBI) 1152
RRIDAB_2882110
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain.