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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CKM-Specific Monoclonal antibody, PBS Only

CKM-Specific Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2G3F6

Cat no : 60177-1-PBS

Synonyms

CKM, 2G3F6, Creatine kinase M-type, CPK-M, CKMM

Validation Data Gallery

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  • Western Blot (WB) analysis of human skeletal muscle tissue using CKM-Specific Monoclonal antibody (60177-1-Ig)

    WB analysis of human skeletal muscle using 60177-1-Ig (same clone as 60177-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60177-1-Ig (CKM-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human skeletal muscle tissue using CKM-Specific Monoclonal antibody (60177-1-Ig)

    WB analysis of human skeletal muscle using 60177-1-Ig (same clone as 60177-1-PBS)

    human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 60177-1-Ig (CKM-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CKM-Specific Monoclonal antibody (60177-1-Ig)

    IHC staining of human liver using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using CKM-Specific Monoclonal antibody (60177-1-Ig)

    IHC staining of mouse testis using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using CKM-Specific Monoclonal antibody (60177-1-Ig)

    IHC staining of rat brain using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using CKM-Specific Monoclonal antibody (60177-1-Ig)

    IHC staining of rat testis using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using CKM-Specific Monoclonal antibody (60177-1-Ig)

    IHC staining of human liver using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse heart tissue using CKM-Specific Monoclonal antibody (60177-1-Ig)

    IHC staining of mouse heart using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 60177-1-Ig (CKM-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse heart tissue using CKM-Specific Monoclonal antibody (60177-1-Ig)

    IF Staining of mouse heart using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CKM-Specific antibody (60177-1-Ig, Clone: 2G3F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using CKM-Specific Monoclonal antibody (60177-1-Ig)

    IF Staining of C2C12 using 60177-1-Ig (same clone as 60177-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using CKM-Specific antibody (60177-1-Ig, Clone: 2G3F6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 60177-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60177-1-PBS targets CKM-Specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name creatine kinase, muscle
Calculated molecular weight 43 kDa
Observed molecular weight 43 kDa, 90 kDa, 130 kDa
GenBank accession numberBC007462
Gene symbol CKM
Gene ID (NCBI) 1158
RRIDAB_10897180
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CKM, also named as CKMM and M-CK, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis and is an important serum marker for myocardial infarction. CKM reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in striated muscle as well as in other tissues, and as a heterodimer with a similar brain isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium. CKM has a calculated molecular mass of 43 kDa, and the 90-kDa and 130-kDa bands could be due to acovalent cross-linking of two and three CKm subunits, respectively (PMID: 20195383).