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  • KD/KO Validated

CKMT1A Polyclonal antibody

CKMT1A Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15346-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF, IP, ELISA

Creatine kinase U-type, mitochondrial, CKMT1B, CKMT1, CKMT, CKMiMi

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inrat heart tissue, human colon tissue, human testis tissue, mouse heart tissue, mouse testis tissue, HEK-293 cells, A549 cells
Positive IP detected inmouse heart tissue
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15346-1-AP targets CKMT1A in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKMT1A fusion protein Ag7583 相同性解析による交差性が予測される生物種
Full Name creatine kinase, mitochondrial 1A
Calculated molecular weight 47 kDa
Observed molecular weight 40-50 kDa
GenBank accession numberBC001926
Gene Symbol CKMT1A
Gene ID (NCBI) 548596
RRIDAB_2081073
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP12532
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.

Protocols

Product Specific Protocols
WB protocol for CKMT1A antibody 15346-1-APDownload protocol
IHC protocol for CKMT1A antibody 15346-1-APDownload protocol
IP protocol for CKMT1A antibody 15346-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Metab

Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.

Authors - Kiran Kurmi
  • KD Validated
humanWB,IHC,IF

Nat Commun

Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation.

Authors - Liang-Jie Wang
  • KD Validated
humanIHC, WB

Respir Res

Mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation.

Authors - Ming Li
humanWB

Mol Oncol

Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis

Authors - Tianwei Chen
mouseIHC

Int J Cancer

Increased serum mitochondrial creatine kinase activity as a risk for hepatocarcinogenesis in chronic hepatitis C patients.

Authors - Kenichiro Enooku
ratIHC

Front Cell Neurosci

Investigations into photoreceptor energy metabolism during experimental retinal detachment

Authors - Glyn Chidlow
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