Validation Data Gallery
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Tested Applications
| Positive WB detected in | mouse heart tissue, human heart tissue, human skeletal muscle tissue, mouse skeletal muscle tissue |
| Positive IHC detected in | mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 8 publications below |
| IHC | See 2 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
Product Information
13207-1-AP targets CKMT2 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CKMT2 fusion protein Ag3971 相同性解析による交差性が予測される生物種 |
| Full Name | creatine kinase, mitochondrial 2 (sarcomeric) |
| Calculated molecular weight | 419 aa, 48 kDa |
| Observed molecular weight | 41-48 kDa |
| GenBank accession number | BC029140 |
| Gene Symbol | CKMT2 |
| Gene ID (NCBI) | 1160 |
| RRID | AB_2081188 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P17540 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CKMT2 antibody 13207-1-AP | Download protocol |
| IHC protocol for CKMT2 antibody 13207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity. | ||
Mol Cell Proteomics Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens | ||
Metabolism Sustained mitochondrial biogenesis is essential to maintain caloric restriction-induced beige adipocytes. | ||
Front Cell Dev Biol Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma | ||
Mol Cell Proteomics Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens. | ||
J Agric Food Chem Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway. |