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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CKS1B Polyclonal antibody (10610-1-AP)

    WB analysis using 10610-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10610-1-AP (CKS1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CKS1B Polyclonal antibody (10610-1-AP)

    WB analysis using 10610-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10610-1-AP (CKS1B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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CKS1B Polyclonal antibody

CKS1B Polyclonal Antibody for WB, ELISA
Cat No. 10610-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

PNAS 143, Cyclin-dependent kinases regulatory subunit 1, ckshs1, CKS-1, CKS1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, HT-29 cells, HepG2 cells, Jurkat cells, SMMC-7721 cells, Y79 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10610-1-AP targets CKS1B in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CKS1B fusion protein Ag0928 相同性解析による交差性が予測される生物種
Full Name CDC28 protein kinase regulatory subunit 1B
Calculated molecular weight 10 kDa
Observed molecular weight10 kDa
GenBank accession numberBC007751
Gene Symbol CKS1B
Gene ID (NCBI) 1163
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP61024
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Cyclin-dependent kinase regulatory subunit 1B (CKS1B), a member of the conserved cyclin kinase subunit 1 (CKS1) protein family, plays an essential role in cell cycling. A large number of studies have shown that CKS1B is associated with the pathogenesis of many human cancers and closely related to drug resistance. (PMID: 33102238)

Protocols

Product Specific Protocols
WB protocol for CKS1B antibody 10610-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols