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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLCC1 Monoclonal antibody

CLCC1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

3C4B4

Cat no : 68853-7-Ig

Synonyms

Mid-1-related chloride channel protein 1, MCLC, KIAA0761, Chloride channel CLIC-like protein 1, chloride channel CLIC like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of hTERT-RPE1 cells using CLCC1 Monoclonal antibody (68853-7-Ig)

    WB analysis of hTERT-RPE1 using 68853-7-Ig

    hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using CLCC1 Monoclonal antibody (68853-7-Ig)

    WB analysis of COLO 320 using 68853-7-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CLCC1 Monoclonal antibody (68853-7-Ig)

    WB analysis using 68853-7-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CLCC1 Monoclonal antibody (68853-7-Ig)

    WB analysis using 68853-7-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68853-7-Ig (CLCC1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inFaDu cells, COLO 320 cells, HepG2 cells, hTERT-RPE1 cells, SiHa cells, SCaBER cells, MG-63 cells, MDA-MB-468 cells, HeLa cells, K-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68853-7-Ig targets CLCC1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CLCC1 fusion protein Ag24682 相同性解析による交差性が予測される生物種
Full Name chloride channel CLIC-like 1
Calculated molecular weight 551 aa, 62 kDa
Observed molecular weight 50-60 kDa
GenBank accession numberBC002939
Gene symbol CLCC1
Gene ID (NCBI) 23155
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLCC1, also known as MCLC, is a transmembrane protein that localizes to intracellular compartments, including endoplasmic reticulum (ER), and it functions as a chloride channel (PMID: 11279057). It has been shown that loss of CLCC1 results in ER Stress, misfolded protein accumulation, and neurodegeneration (PMID: 25698737). CLCC1 forms homomultimer in the ER membrane and is a pore-forming component of an anion channel. Studies found that disruption of ER ion homeostasis maintained by CLCC1 is one of the causes of neurodegenerative diseases, such as ALS (amyotrophic lateral sclerosis) (PMID: 37142673).

Protocols

Product Specific Protocols
WB protocol for CLCC1 antibody 68853-7-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols