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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLCN7 Polyclonal antibody (29230-1-AP)

    WB analysis using 29230-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29230-1-AP (CLCN7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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CLCN7 Polyclonal antibody

CLCN7 Polyclonal Antibody for WB, ELISA
Cat No. 29230-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

OPTA2, H(+)/Cl(-) exchange transporter 7, ClC-7, CLC7, CLC 7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse kidney tissue, mouse small intestine mouse small intestine, rat small intestine rat small intestine

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29230-1-AP targets CLCN7 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLCN7 fusion protein Ag29882 相同性解析による交差性が予測される生物種
Full Name chloride channel 7
Calculated molecular weight 805 aa, 89 kDa
Observed molecular weight70 kDa
GenBank accession numberBC012737
Gene Symbol CLCN7
Gene ID (NCBI) 1186
RRIDAB_3669678
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP51798
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The intracellular CLC chloride channel family members ClC-3, ClC-4, and ClC-5 have been proposed as the primary endosomal chloride conductance providers. ClC-7 is expressed in late endosomes and lysosomes, but there is disagreement in the literature regarding its contribution to acidification. More recently, the cystic fibrosis (CF) transmembrane conductance regulator chloride channel (CFTR) was proposed as the specific counter-ion conductance necessary for lysosomal acidification in alveolar macrophages. (PMID: 20566682)

Protocols

Product Specific Protocols
WB protocol for CLCN7 antibody 29230-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols