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  • Featured Product
  • KD/KO Validated

Claudin 1 Polyclonal antibody

Claudin 1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (5)

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13050-1-AP

Synonyms

Caudin 1, CLDN1, SEMP1, ILVASC, CLD1

Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using Claudin 1 Polyclonal antibody (13050-1-AP)

    WB analysis of A431 using 13050-1-AP

    WB result of Claudin 1 antibody (13050-1-AP; 1:1500; incubated at room temperature for 1.5 hours) with sh-Control and sh-Claudin 1 transfected A431 cells.

  • Western Blot (WB) analysis of various lysates using Claudin 1 Polyclonal antibody (13050-1-AP)

    WB analysis using 13050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Claudin 1 Polyclonal antibody (13050-1-AP)

    WB analysis using 13050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using Claudin 1 Polyclonal antibody (13050-1-AP)

    WB analysis using 13050-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    WB analysis of mouse liver using 13050-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP (Claudin 1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    WB analysis of mouse thymus using 13050-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13050-1-AP (CLDN1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of human skin cancer using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of human skin cancer using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of human cervical cancer using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of human cervical cancer using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of human malignant melanoma using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human malignant melanoma tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of human malignant melanoma using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded human malignant melanoma tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of human oesophagus cancer using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of mouse skin using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse skin tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of mouse skin using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Claudin 1 Polyclonal antibody (13050-1-AP)

    IHC staining of human oesophagus cancer using 13050-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 13050-1-AP (Claudin 1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using Claudin 1 Polyclonal antibody (13050-1-AP)

    IF Staining of HUVEC using 13050-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HUVEC cells using Claudin 1 antibody (13050-1-AP) at dilution of 1:2000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using Claudin 1 Polyclonal antibody (13050-1-AP)

    IF Staining of MDCK using 13050-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 13050-1-AP (Claudin 1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA431 cells, mouse liver tissue, mouse skin tissue, mouse thymus tissue, A549 cells, HUVEC cells, rat skin tissue
Positive IHC detected inhuman cervical cancer tissue, human skin cancer tissue, mouse skin tissue, human oesophagus cancer tissue, human malignant melanoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHUVEC cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13050-1-AP targets Claudin 1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, bovine, sheep, goat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 1 fusion protein Ag3713 相同性解析による交差性が予測される生物種
Full Name claudin 1
Calculated molecular weight 211 aa, 23 kDa
Observed molecular weight 20-23 kDa
GenBank accession numberBC012471
Gene symbol Claudin 1
Gene ID (NCBI) 9076
RRIDAB_2079881
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. Claudin-1 is an integral membrane protein expressed primarily in keratinocytes and normal mammary epithelial cells. Claudin 1 forms tight junctions with other claudin proteins and plays an important role in the intestinal epithelial barrier.

Protocols

Product Specific Protocols
WB protocol for Claudin 1 antibody 13050-1-APDownload protocol
IHC protocol for Claudin 1 antibody 13050-1-APDownload protocol
IF protocol for Claudin 1 antibody 13050-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Cell Host Microbe

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Authors - Zhenyu Tian
View Article
human,mouseIHC

Clin Cancer Res

ZIP4 Promotes Pancreatic Cancer Progression by Repressing ZO-1 and claudin-1 through a ZEB1-Dependent Transcriptional Mechanism.

Authors - Mingyang Liu
View Article
humanIF

EMBO Rep

O-glycan initiation directs distinct biological pathways and controls epithelial differentiation.

Authors - Ieva Bagdonaite
View Article
ratIHC

Mater Today Bio

Intracellular hydrogelation of macrophage conjugated probiotics for hitchhiking delivery and combined treatment of colitis

Authors - Jingzhe Wang
View Article
mouseIF

Int J Biol Macromol

Glucose-modified BSA/procyanidin C1 NPs penetrate the blood-brain barrier and alleviate neuroinflammation in Alzheimer's disease models

Authors - Linyan Duan
View Article
humanWB

PLoS Pathog

Signal Peptidase Complex Subunit 1 Participates in the Assembly of Hepatitis C Virus through an Interaction with E2 and NS2.

Authors - Suzuki Ryosuke R
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

James (Verified Customer) (01-06-2022)

Works good. Nice image

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Gut
Claudin 1 Antibody Immunohistochemistry validation (1:100 dilution) in Gut (Cat no:13050-1-AP)
FH

Joshua (Verified Customer) (07-27-2019)

Cells differentiated at air-liquid interface. Cells fixed in 4% paraformaldehyde and stained at 4C overnight. Moderate staining with background. Staining is a mix of junctional (bright) and cytosolic (dim).

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human Keratinocytes
Claudin 1 Antibody Immunofluorescence, validation (1:500 dilution) in Human Keratinocytes (Cat no:13050-1-AP)