Claudin 11 Polyclonal antibody
Claudin 11 Polyclonal Antibody for WB, IF, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse and More (1)
Applications
WB, IF, IHC, ELISA
Conjugate
Unconjugated
Cat no : 12152-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human testis tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IF | See 1 publications below |
Product Information
12152-1-AP targets Claudin 11 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Claudin 11 fusion protein Ag2764 相同性解析による交差性が予測される生物種 |
| Full Name | claudin 11 |
| Calculated molecular weight | 207 aa, 22 kDa |
| Observed molecular weight | 22 kDa |
| GenBank accession number | BC013577 |
| Gene symbol | Claudin 11 |
| Gene ID (NCBI) | 5010 |
| RRID | AB_10694425 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Claudin 11 antibody 12152-1-AP | Download protocol |
| IHC protocol for Claudin 11 antibody 12152-1-AP | Download protocol |
| IF protocol for Claudin 11 antibody 12152-1-AP | Download protocol |
| FC protocol for Claudin 11 antibody 12152-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Endocrinol Cardiotonic steroid ouabain stimulates expression of blood-testis barrier proteins claudin-1 and -11 and formation of tight junctions in Sertoli cells. | ||
Environ Sci Pollut Res Int Polystyrene microplastics induce blood-testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats. | ||
J Physiol Biochem α2,6-Sialylation promotes hepatocellular carcinoma cells migration and invasion via enhancement of nSmase2-mediated exosomal miRNA sorting | ||
Sci Rep Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis. |
