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Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLDN16 Recombinant antibody (82954-1-RR)

    IHC staining of mouse kidney using 82954-1-RR (same clone as 82954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82954-1-RR (CLDN16 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82954-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using CLDN16 Recombinant antibody (82954-1-RR)

    IHC staining of mouse kidney using 82954-1-RR (same clone as 82954-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 82954-1-RR (CLDN16 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82954-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CLDN16 Recombinant antibody (82954-1-RR)

    IF Staining of HEK-293 using 82954-1-RR (same clone as 82954-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CLDN16 antibody (82954-1-RR, Clone: 230193G3 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 82954-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CLDN16 Recombinant antibody, PBS Only (82954-1-PBS)

    IF Staining of HEK-293 using 82954-1-PBS

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using CLDN16 antibody (82954-1-PBS, Clone: 230193G3 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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CLDN16 Recombinant antibody, PBS Only

CLDN16 Uni-rAbTM Recombinant Antibody for IHC, IF/ICC, Indirect ELISA
Cat No. 82954-1-PBS
Clone No.230193G3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Synonyms

PCLN-1, PCLN1, Paracellin-1, Claudin-16, 230193G3

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82954-1-PBS targets CLDN16 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLDN16 fusion protein Ag26531 相同性解析による交差性が予測される生物種
Full Name claudin 16
Calculated molecular weight 305 aa, 34 kDa
GenBank accession numberBC069682
Gene Symbol CLDN16
Gene ID (NCBI) 10686
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y5I7
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Protocols

Product Specific Protocols
IF protocol for CLDN16 antibody 82954-1-PBSDownload protocol
Standard Protocols
Click here to view our Standard Protocols