Claudin 18 Monoclonal antibody
Claudin 18 Monoclonal Antibody for IP, IHC, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, mouse
Applications
IP, IF, IHC, ELISA
Conjugate
Unconjugated
CloneNo.
5G7F2
Cat no : 66167-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive IP detected in | mouse stomach tissue |
Positive IHC detected in | human stomach tissue, human pancreas cancer tissue, human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
66167-1-Ig targets Claudin 18 in IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Claudin 18 fusion protein Ag15517 相同性解析による交差性が予測される生物種 |
Full Name | claudin 18 |
Calculated molecular weight | 261 aa, 28 kDa |
Observed molecular weight | 29 kDa |
GenBank accession number | BC146668 |
Gene symbol | CLDN18 |
Gene ID (NCBI) | 51208 |
RRID | AB_2881563 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Claudin 18 (CLDN18) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 11585919; 18036336). Claudins exhibit specific patterns of expression in different tissues (PMID: 15447685). CLDN18 is specifically expressed in the stomach and lung. CLDN18 has two alternatively spliced variants, CLDN18.1 and CLDN18.2. CLDN18.2 is a highly selective gastric lineage marker that determines the gastric phenotype in a neoplastic condition, whereas CLDN18.1 is lung specific (PMID: 11585919; 21832145). Altered CLDN18 expression has been reported in various human malignancies, including gastric cancer, lung adenocarcinoma, and pancreatic neoplasm (PMID: 17459057; 22076167; 21832145). This antibody can recognize both CLDN18.1 and CLDN18.2.
Protocols
Product Specific Protocols | |
---|---|
IHC protocol for Claudin 18 antibody 66167-1-Ig | Download protocol |
IP protocol for Claudin 18 antibody 66167-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mucosal Immunol Airway epithelium IgE-FcεRI Cross-Link Induces Epithelial Barrier Disruption in Severe T2-high Asthma | ||
World J Gastrointest Pathophysiol Visualization of sphingolipids and phospholipids in the fundic gland mucosa of human stomach using imaging mass spectrometry. |