Validation Data Gallery
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Tested Applications
| Positive IP detected in | mouse large intestine tissue |
| Positive IHC detected in | human breast cancer tissue, mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 12 publications below |
| IF | See 14 publications below |
Product Information
16456-1-AP targets Claudin 3 in IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, sheep |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Claudin 3 fusion protein Ag9411 相同性解析による交差性が予測される生物種 |
| Full Name | claudin 3 |
| Calculated molecular weight | 220 aa, 23 kDa |
| Observed molecular weight | 20-24 kDa |
| GenBank accession number | BC016056 |
| Gene Symbol | Claudin 3 |
| Gene ID (NCBI) | 1365 |
| RRID | AB_10733226 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15551 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Claudin 3 antibody 16456-1-AP | Download protocol |
| IP protocol for Claudin 3 antibody 16456-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Aging Cell Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts. | ||
EMBO Mol Med Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation. | ||
Sci China Life Sci Longitudinal gut fungal alterations and potential fungal biomarkers for the progression of primary liver disease | ||
J Hazard Mater Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles. | ||
Environ Int Translocation of transition metal oxide nanoparticles to breast milk and offspring: The necessity of bridging mother-offspring-integration toxicological assessments. | ||
Biochem Pharmacol Inhibition of S100A9 alleviates neurogenic pulmonary edema after subarachnoid hemorrhage |