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  • Featured Product
  • KD/KO Validated

Claudin 4-specific Polyclonal antibody

Claudin 4-specific Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 16195-1-AP

Synonyms

CLDN4, Claudin 4, CPE-R, CPER, CPE receptor

Validation Data Gallery

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  • Western Blot (WB) analysis of MDA-MB-453s cells using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    WB analysis of MDA-MB-453s using 16195-1-AP

    MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse small intestine tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    WB analysis of mouse small intestine using 16195-1-AP

    mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16195-1-AP (CLDN4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    WB analysis of human placenta using 16195-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16195-1-AP (CLDN4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human placenta tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IP experiment of human placenta using 16195-1-AP

    IP result of anti-Claudin 4-specific (IP:16195-1-AP, 4ug; Detection:16195-1-AP 1:300) with human placenta tissue lysate 4400ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human breast cancer using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human breast cancer using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human ovary tumor using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human ovary tumor using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human breast cancer using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human breast cancer using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human ovary tumor using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human ovary tumor using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human lung cancer using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IHC staining of human lung cancer using 16195-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IF Staining of human breast cancer using 16195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Claudin 4-specific antibody (16195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Claudin 4-specific Polyclonal antibody (16195-1-AP)

    IF Staining of human breast cancer using 16195-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Claudin 4-specific antibody (16195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inMDA-MB-453s cells, mouse small intestine tissue, human placenta tissue
Positive IP detected inhuman placenta tissue
Positive IHC detected inhuman breast cancer tissue, human ovary tumor tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16195-1-AP targets Claudin 4-specific in WB, IHC, IF-P, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name claudin 4
Calculated molecular weight 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberNM_001305
Gene symbol Claudin 4
Gene ID (NCBI) 1364
RRIDAB_2082969
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kDa) proteins with very similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against Claudin 4. Claudin 4 is overexpressed in a variety of cancers. It can be used as a predictor of recurrent malignant pleural effusion in patients with advanced non-small cell lung cancer (PMID: 38629624).

Protocols

Product Specific Protocols
WB protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
IHC protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
IF protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
IP protocol for Claudin 4-specific antibody 16195-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIHC,IF

Cell Metab

Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes

Authors - Junpeng Yang
View Article
ratWB

Gut Microbes

S-amlodipine induces liver inflammation and dysfunction through the alteration of intestinal microbiome in a rat model

Authors - Xinxin Liu
View Article
mouse,ratWB,IHC

J Hazard Mater

Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.

Authors - Junrong Wu
View Article
humanWB

Free Radic Biol Med

Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.

Authors - Restituto Tocmo
View Article
humanWB, RIP

Cancers (Basel)

Targeting PVT1 Exon 9 Re-Expresses Claudin 4 Protein and Inhibits Migration by Claudin-Low Triple Negative Breast Cancer Cells.

Authors - Fayola Levine
View Article
ratWB

EBioMedicine

Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis.

Authors - Yingchun Luo
View Article