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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Claudin 7 Polyclonal antibody

Claudin 7 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 29795-1-AP

Synonyms

CEPTRL2, Claudin 7, CLDN 7, CLDN7, CPETRL2, Hs.84359

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Claudin 7 Polyclonal antibody (29795-1-AP)

    IHC staining of human oesophagus cancer using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using Claudin 7 Polyclonal antibody (29795-1-AP)

    IHC staining of human oesophagus cancer using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Claudin 7 Polyclonal antibody (29795-1-AP)

    IHC staining of human colon using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Claudin 7 Polyclonal antibody (29795-1-AP)

    IHC staining of human colon using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Claudin 7 Polyclonal antibody (29795-1-AP)

    IHC staining of mouse colon using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using Claudin 7 Polyclonal antibody (29795-1-AP)

    IHC staining of mouse colon using 29795-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 29795-1-AP (Claudin 7 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive IHC detected inhuman colon tissue, mouse colon tissue, human oesophagus cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

29795-1-AP targets Claudin 7 in IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Claudin 7 fusion protein Ag31706 相同性解析による交差性が予測される生物種
Full Name claudin 7
Calculated molecular weight22.4 kDa
Observed molecular weight23 kDa
GenBank accession numberBC001055
Gene symbol Claudin 7
Gene ID (NCBI) 1366
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 27 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Protocols

Product Specific Protocols
IHC protocol for Claudin 7 antibody 29795-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

J Agric Food Chem

Lacticaseibacillus rhamnosus KY16 Improves Depression by Promoting Intestinal Secretion of 5-HTP and Altering the Gut Microbiota

Authors - Shicai Xie
View Article