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Validation Data Gallery

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  • Western Blot (WB) analysis of BxPC-3 cells using CLEC10A/CD301 Polyclonal antibody (13590-1-AP)

    WB analysis of BxPC-3 using 13590-1-AP

    BxPC-3 cells were subjected to SDS PAGE followed by western blot with 13590-1-AP (CLEC10A/CD301 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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CLEC10A/CD301 Polyclonal antibody

CLEC10A/CD301 Polyclonal Antibody for WB, ELISA
Cat No. 13590-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CLEC10A, CD301, C-type lectin superfamily member 14, C-type lectin domain family 10 member A, CLECSF14

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13590-1-AP targets CLEC10A/CD301 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC10A/CD301 fusion protein Ag4490 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 10, member A
Calculated molecular weight 316 aa, 35 kDa
Observed molecular weight 32 kDa, 28 kDa
GenBank accession numberBC039011
Gene Symbol CLEC10A
Gene ID (NCBI) 10462
RRIDAB_2083131
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IUN9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C-type lectin domain family 10 member A (CLEC10A), also known as CD301, macrophage galactose-type C-type lectin (MGL), DC-asialoglyco protein receptor (DC-ASGP-R) or human macrophage lectin (HML), is a type II transmembrane glycoprotein having a calcium-dependent carbohydrate recognition domain (PMID: 8598452; 11919201; 11698450). CLEC10A is expressed exclusively by myeloid professional antigen-presenting cells such as immature dendritic cells (DCs) and alternatively activated macrophages (PMID: 16998493). CLEC10A is a marker for human CD1c+ DCs (cDC2) (PMID: 29755453). It functions as an endocytic receptor for glycosylated antigens (PMID: 11919201).

Protocols

Product Specific Protocols
WB protocol for CLEC10A/CD301 antibody 13590-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols