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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLEC10A Recombinant antibody (84748-1-RR)

    WB analysis using 84748-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84748-1-RR (CLEC10A antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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CLEC10A/CD301 Recombinant antibody

CLEC10A/CD301 Recombinant Antibody for WB, ELISA
Cat No. 84748-1-RR
Clone No.241857C3

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

CLEC10A, C-type lectin domain family 10 member A, CLECSF14, CLECSF13, CD301

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inK-562 cells, Raji cells, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84748-1-RR targets CLEC10A/CD301 in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 10, member A
Calculated molecular weight 316 aa, 35 kDa
Observed molecular weight35 kDa
GenBank accession numberBC039011
Gene Symbol CLEC10A
Gene ID (NCBI) 10462
RRIDAB_3672131
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IUN9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C-type lectin domain family 10 member A (CLEC10A), also known as CD301, macrophage galactose-type C-type lectin (MGL), DC-asialoglyco protein receptor (DC-ASGP-R) or human macrophage lectin (HML), is a type II transmembrane glycoprotein having a calcium-dependent carbohydrate recognition domain (PMID: 8598452; 11919201; 11698450). CLEC10A is expressed exclusively by myeloid professional antigen-presenting cells such as immature dendritic cells (DCs) and alternatively activated macrophages (PMID: 16998493). CLEC10A is a marker for human CD1c+ DCs (cDC2) (PMID: 29755453). It functions as an endocytic receptor for glycosylated antigens (PMID: 11919201).

Protocols

Product Specific Protocols
WB protocol for CLEC10A/CD301 antibody 84748-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols