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Validation Data Gallery

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  • Western Blot (WB) analysis of U-937 cells using CLEC12A Polyclonal antibody (28627-1-AP)

    WB analysis of U-937 using 28627-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 28627-1-AP (CLEC12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CLEC12A Polyclonal antibody (28627-1-AP)

    WB analysis using 28627-1-AP

    Human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 28627-1-AP (CLEC12A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of U-937 cells using CLEC12A Polyclonal antibody (28627-1-AP)

    IF Staining of U-937 using 28627-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells without permeabilization using CLEC12A antibody (28627-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

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CLEC12A Polyclonal antibody

CLEC12A Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 28627-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

C-type lectin domain family 12 member A, CLL-1, CLL1, CLL 1, CD371

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inU-937 cells, human peripheral blood leukocyte
Positive IF/ICC detected inU-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28627-1-AP targets CLEC12A in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC12A fusion protein Ag27864 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 12, member A
Calculated molecular weight 31 kDa
Observed molecular weight55-75 kDa
GenBank accession numberBC063424
Gene Symbol CLEC12A
Gene ID (NCBI) 160364
RRIDAB_3086071
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5QGZ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).

Protocols

Product Specific Protocols
WB protocol for CLEC12A antibody 28627-1-APDownload protocol
IF protocol for CLEC12A antibody 28627-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols