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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Anti-Human CLEC12A Rabbit Recombinant Antibody, PBS Only

CLEC12A Recombinant Antibody for FC

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Conjugate

Unconjugated

CloneNo.

240791B7

Cat no : 98083-1-PBS

Synonyms

CLL-1, CLL1, CLL 1, CD371, C type lectin like molecule 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR)

    FC experiment of human peripheral blood leukocytes using 98083-1-RR (same clone as 98083-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7), or Isotype Control and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD16. Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed. This data was developed using the same antibody clone with 98083-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR)

    FC experiment of human peripheral blood leukocytes using 98083-1-RR (same clone as 98083-1-PBS)

    1x10^6 human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7)(red), or 0.25 ug Isotype Control(blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining.Cells were not fixed. This data was developed using the same antibody clone with 98083-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of human peripheral blood leukocytes using Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR)

    FC experiment of human peripheral blood leukocytes using 98083-1-RR (same clone as 98083-1-PBS)

    1x10^6 FC receptor blocked human peripheral blood leukocytes were surface stained with 0.25 ug Anti-Human CLEC12A Rabbit Recombinant Antibody (98083-1-RR, Clone:240791B7) and PE-Conjugated Goat Anti-Rabbit IgG(H+L), and 0.25 ug CoraLite® Plus 647 Anti-Human CD16 (3G8) Mouse IgG2a Recombinant Antibody (CL647-65612, Clone: 3G8), and 0.25 ug Isotype Control. Cells were not fixed. This data was developed using the same antibody clone with 98083-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 98083-1-RR

    Affinity and Kinetic Characterization of 98083-1-RR

    Biolayer interferometry (BLl) kinetic assays of 98083-1-RR against Human CLEC12A were performed. The affinity constant is 0.524 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 98083-1-PBS is CLEC12A Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 12, member A
Calculated molecular weight31 kDa
GenBank accession numberBC126291
Gene symbol CLEC12A
Gene ID (NCBI) 160364
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).