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Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using CLEC14A Polyclonal antibody (23964-1-AP)

    WB analysis of human heart using 23964-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using CLEC14A Polyclonal antibody (23964-1-AP)

    WB analysis using 23964-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse skeletal muscle tissue using CLEC14A Polyclonal antibody (23964-1-AP)

    WB analysis of mouse skeletal muscle using 23964-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23964-1-AP (CLEC14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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CLEC14A Polyclonal antibody

CLEC14A Polyclonal Antibody for WB, ELISA
Cat No. 23964-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

UNQ236/PRO269, EGFR-5, C-type lectin domain family 14 member A, CEG1, C14orf27

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse brain tissue, mouse skeletal muscle tissue, human heart tissue, rat brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23964-1-AP targets CLEC14A in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC14A fusion protein Ag21081 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 14, member A
Calculated molecular weight 490 aa, 52 kDa
Observed molecular weight 52 kDa, 100 kDa
GenBank accession numberBC031567
Gene Symbol CLEC14A
Gene ID (NCBI) 161198
RRIDAB_2879377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ86T13
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLEC14A is a single-pass type I transmembrane protein whose extracellular domain consists of a C-type lectin-like domain (CTLD), a series of EGF-like domains, and a sushi-like domain (PMID: 23644659). CLEC14A is a member of the C-type lectin domain superfamily that also includes endosialin, thrombomodulin and CD93 (PMID: 16336259). It has been reported that CLEC14A is specifically expressed in endothelial cells and mediates cell to cell adhesion (PMID: 21095181). CLEC14A may be involved in tumor angiogenesis (PMID: 21706054; 23644659).

Protocols

Product Specific Protocols
WB protocol for CLEC14A antibody 23964-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC

FASEB J

CLEC14A protects against podocyte injury in mice with adriamycin nephropathy.

Authors - Zeyu Su
View Article