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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLEC1A Polyclonal antibody

CLEC1A Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13394-1-AP

Synonyms

C type lectin like receptor 1, CLEC 1, CLEC1, CLEC1A

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLEC1A Polyclonal antibody (13394-1-AP)

    WB analysis using 13394-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13394-1-AP (CLEC1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    WB analysis of mouse thymus using 13394-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 13394-1-AP (CLEC1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    IHC staining of mouse lung using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13394-1-AP (CLEC1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse lung tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    IHC staining of mouse lung using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 13394-1-AP (CLEC1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    IHC staining of human lung cancer using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13394-1-AP (CLEC1A antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using CLEC1A Polyclonal antibody (13394-1-AP)

    IHC staining of human lung cancer using 13394-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 13394-1-AP (CLEC1A antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

Positive WB detected inmouse lung tissue, mouse thymus tissue, rat lung tissue
Positive IHC detected inmouse lung tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

13394-1-AP targets CLEC1A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC1A fusion protein Ag4058 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 1, member A
Calculated molecular weight 280 aa, 32 kDa
Observed molecular weight 50 kDa, 32 kDa
GenBank accession numberBC039072
Gene symbol CLEC1A
Gene ID (NCBI) 51267
RRIDAB_2083443
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLEC1A (C-type lectin domain family 1 member A) is a single-pass type II membrane protein that contains one C-type lectin domain. Members of the C-type lectin domain family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. CLEC1A is expressed preferentially in dendritic cells and plays a role in regulating dendritic cell function (PMID: 10671229).

Protocols

Product Specific Protocols
WB protocol for CLEC1A antibody 13394-1-APDownload protocol
IHC protocol for CLEC1A antibody 13394-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

EMBO Mol Med

Histidine-rich glycoprotein modulates neutrophils and thrombolysis-associated hemorrhagic transformation

Authors - Wei Jiang
View Article
mouse,humanIF

Invest Ophthalmol Vis Sci

CLEC-1 Acts as a Negative Regulator of Dectin-1 Induced Host Inflammatory Response Signature in Aspergillus fumigatus Keratitis.

Authors - Guoqiang Zhu
View Article