Validation Data Gallery
Tested Applications
| Positive WB detected in | human peripheral blood platelets |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
12814-1-AP targets CLEC2/CLEC1B in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | CLEC2/CLEC1B fusion protein Ag3512 相同性解析による交差性が予測される生物種 |
| Full Name | C-type lectin domain family 1, member B |
| Calculated molecular weight | 27 kDa |
| Observed molecular weight | 32-38 kDa |
| GenBank accession number | BC029554 |
| Gene Symbol | CLEC2 |
| Gene ID (NCBI) | 51266 |
| RRID | AB_3085400 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9P126 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
C-type lectin-like receptor 2 (CLEC2, also known as CLEC1B) is a type II transmembrane receptor of the C-type lectin superfamily, which are characterized by one or more C-type lectin-like domains (CTLDs) (PMID: 10671229; 34177942). CLEC2 contains a single YXXL/hemi-ITAM (immuno-receptor tyrosine-based activation motif) within its cytoplasmic domain. CLEC-2 is highly expressed on platelets and megakaryocytes and at low levels on other hematopoietic cells, including dendritic cells, neutrophils, monocytes, and macrophages (PMID: 21728173). The first identified ligand for CLEC2 was the platelet-aggregating snake venom protein rhodocytin, and podoplanin has been established as an endogenous ligand for CLEC2 (PMID: 26151067). CLEC2 is involved in thrombosis/hemostasis, tumor metastasis, and lymphangiogenesis (PMID: 20525685). CLEC2 can be glycosylated and has been detected as 32- and 40-kDa forms in platelets with varying degrees of glycosylation (PMID: 16174766; 25150298; 21781241).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CLEC2/CLEC1B antibody 12814-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
EMBO Mol Med Histidine-rich glycoprotein modulates neutrophils and thrombolysis-associated hemorrhagic transformation | ||
Int J Gen Med CLEC1B is a Promising Prognostic Biomarker and Correlated with Immune Infiltration in Hepatocellular Carcinoma. |