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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CLEC2D Polyclonal antibody (13188-1-AP)

    WB analysis using 13188-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13188-1-AP (CLEC2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using CLEC2D Polyclonal antibody (13188-1-AP)

    WB analysis of Jurkat using 13188-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 13188-1-AP (CLEC2D antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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CLEC2D Polyclonal antibody

CLEC2D Polyclonal Antibody for WB, ELISA
Cat No. 13188-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CLAX, CLEC2D, Lectin like NK cell receptor, Lectin like transcript 1, LLT 1, LLT1, OCIL, Osteoclast inhibitory lectin

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inDaudi cells, Jurkat cells, Saos-2 cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13188-1-AP targets CLEC2D in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC2D fusion protein Ag3862 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 2, member D
Calculated molecular weight 154 aa, 18 kDa
Observed molecular weight 33 kDa
GenBank accession numberBC019883
Gene Symbol CLEC2D
Gene ID (NCBI) 29121
RRIDAB_2877920
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UHP7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CLEC2D (C-type lectin domain family 2 member D), also known as LLT1 (lectin-like transcript 1) or OCIL (Osteoclast inhibitory lectin), is a single-pass type II membrane protein expressed by hematopoietic cells and osteoblasts (PMID: 21930700; 14753741). Its expression was found to be tightly correlated with activation on T cells, NK cells, B cells, and DCs (PMID: 21930700). CLEC2D is a ligand for the human NKR-P1A (CD161) receptor and their interaction differentially regulates NK- and T-cell functions (PMID: 16339513, 16339512). It inhibits the formation and function of osteoclasts and modulates the release of interferon-gamma by human NK cells (PMID:14753741; 20415786).

Protocols

Product Specific Protocols
WB protocol for CLEC2D antibody 13188-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols