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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CLEC3B Polyclonal antibody

CLEC3B Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 20311-1-AP

Synonyms

TNA, TN, Tetranectin, Plasminogen kringle 4-binding protein, C-type lectin domain family 3 member B

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using CLEC3B Polyclonal antibody (20311-1-AP)

    WB analysis of human plasma using 20311-1-AP

    human plasma were subjected to SDS PAGE followed by western blot with 20311-1-AP (CLEC3B Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of human plasma using CLEC3B Polyclonal antibody (20311-1-AP)

    IP experiment of human plasma using 20311-1-AP

    IP result of anti-CLEC3B (IP:20311-1-AP, 4ug; Detection:20311-1-AP 1:300) with human plasma lysate 4000ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLEC3B Polyclonal antibody (20311-1-AP)

    IF Staining of HeLa using 20311-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CLEC3B antibody (20311-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman plasma tissue
Positive IP detected inhuman plasma tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20311-1-AP targets CLEC3B in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLEC3B fusion protein Ag14147 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 3, member B
Calculated molecular weight 202 aa, 22 kDa
Observed molecular weight 22 kDa
GenBank accession numberBC011024
Gene symbol CLEC3B
Gene ID (NCBI) 7123
RRIDAB_2878670
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

C-Type Lectin Domain Family 3 Member B (CLEC3B) is a transmembrane Ca2+-binding protein, locating in cell plasma, extracellular matrix and exosomes. Downregulation of CLEC3B has been reported in various diseases. It was demonstrated in CLEC3B-deficient mice that the absence of CLEC3B impeded the mineralization process in osteogenesis. (PMID: 31477130)

Protocols

Product Specific Protocols
WB protocol for CLEC3B antibody 20311-1-APDownload protocol
IF protocol for CLEC3B antibody 20311-1-APDownload protocol
IP protocol for CLEC3B antibody 20311-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols