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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Dectin-1 Polyclonal antibody

Dectin-1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 22809-1-AP

Synonyms

Beta glucan receptor, BGR, CD369, CLEC7A, CLECSF12, DC associated C type lectin 1, Dectin 1, DECTIN1, Dectin-1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Dectin-1 Polyclonal antibody (22809-1-AP)

    WB analysis using 22809-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 22809-1-AP (Dectin-1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHL-60 cells, THP-1 cells, U-937 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22809-1-AP targets Dectin-1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Dectin-1 fusion protein Ag18924 相同性解析による交差性が予測される生物種
Full Name C-type lectin domain family 7, member A
Calculated molecular weight 247 aa, 28 kDa
Observed molecular weight45 kDa
GenBank accession numberBC013385
Gene symbol Dectin-1
Gene ID (NCBI) 64581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Dectin-1, also known as CLEC7A or CD369, is a type II membrane receptor consisting of an extracellular C-terminal C-type lectin domain, a short stalk region, a single transmembrane domain and a short N-terminal cytoplasmic tail, which contains an immunoreceptor tyrosine-based activation motif (PMID: 10779524; 21612412). Dectin-1 is expressed on monocytes, macrophages, neutrophils, dendritic cells and a subpopulation of T cells (PMID: 12244185). Dectin-1 plays a role in the innate immune response. It functions as a pattern-recognition receptor and recognizes a variety of β-glucans from fungi, plants and bacteria. Dectin-1 may also participate in orchestrating the adaptive immune response (PMID: 21612412). The apparent molecular weight is larger than the calculated molecular weight due to glycosylation (PMID: 10779524).

Protocols

Product Specific Protocols
WB protocol for Dectin-1 antibody 22809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols