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Validation Data Gallery

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  • Western Blot (WB) analysis of HL-60 cells using CLIC1 Polyclonal antibody (14545-1-AP)

    WB analysis of HL-60 using 14545-1-AP

    HL-60 cells were subjected to SDS PAGE followed by western blot with 14545-1-AP (CLIC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using CLIC1 Polyclonal antibody (14545-1-AP)

    WB analysis of HeLa using 14545-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 14545-1-AP (CLIC1 Antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using CLIC1 Polyclonal antibody (14545-1-AP)

    IP experiment of HeLa using 14545-1-AP

    IP result of anti-CLIC1 (IP:14545-1-AP, 4ug; Detection:14545-1-AP 1:300) with HeLa cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CLIC1 Polyclonal antibody (14545-1-AP)

    IHC staining of human small intestine using 14545-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human small intestine tissue using CLIC1 Polyclonal antibody (14545-1-AP)

    IHC staining of human small intestine using 14545-1-AP

    Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CLIC1 Polyclonal antibody (14545-1-AP)

    IHC staining of human colon using 14545-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using CLIC1 Polyclonal antibody (14545-1-AP)

    IHC staining of human colon using 14545-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CLIC1 Polyclonal antibody (14545-1-AP)

    IHC staining of human pancreas using 14545-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using CLIC1 Polyclonal antibody (14545-1-AP)

    IHC staining of human pancreas using 14545-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14545-1-AP (CLIC1 Antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CLIC1 Polyclonal antibody (14545-1-AP)

    IF Staining of HepG2 using 14545-1-AP

    Immunofluorescent analysis of HepG2 cells, using CLIC1 antibody 14545-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CLIC1 Polyclonal antibody (14545-1-AP)

    IF Staining of HeLa using 14545-1-AP

    Immunofluorescent analysis of Hela cells, using CLIC1 antibody 14545-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  • Featured Product
  • KD/KO Validated

CLIC1 Polyclonal antibody

CLIC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 14545-1-AP
Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

NCC27, G6

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHL-60 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman small intestine tissue, human colon tissue, human pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, Hela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14545-1-AP targets CLIC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CLIC1 fusion protein Ag6049 相同性解析による交差性が予測される生物種
Full Name chloride intracellular channel 1
Calculated molecular weight 27 kDa
Observed molecular weight 27-32 kDa
GenBank accession numberBC064527
Gene Symbol CLIC1
Gene ID (NCBI) 1192
RRIDAB_2081809
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00299
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for CLIC1 antibody 14545-1-APDownload protocol
IHC protocol for CLIC1 antibody 14545-1-APDownload protocol
IF protocol for CLIC1 antibody 14545-1-APDownload protocol
IP protocol for CLIC1 antibody 14545-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Oxid Med Cell Longev

Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.

Authors - Songhan Li
View Article
humanWB

Transl Oncol

Chloride intracellular channel 1 promotes esophageal squamous cell carcinoma proliferation via mTOR signalling

Authors - Huiwu Geng
  • KD Validated
View Article

Cell Biochem Biophys

CLIC1 Inhibition Protects Against Cellular Senescence and Endothelial Dysfunction Via the Nrf2/HO-1 Pathway.

Authors - Dezhao Lu
View Article
humanIHC

Anticancer Res

Chloride Intracellular Channel 1 Expression Is Associated With Poor Prognosis of Lung Adenocarcinoma.

Authors - Yuichiro Yasuda
View Article
mouseWB,IHC

Technol Cancer Res Treat

CLIC1 Drives Angiogenesis in Hepatocellular Carcinoma by Modulating VEGFA.

Authors - Xuyong Wei
  • KO Validated
View Article
humanWB,IHC

Breast Cancer (Dove Med Press)

Chloride Intracellular Channel 1 is a Potential Biomarker for Breast Cancer

Authors - Jinwen Xia
View Article