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Validation Data Gallery

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  • Western Blot (WB) analysis of JAR cells using CLIC3 Recombinant antibody (84759-4-RR)

    WB analysis of JAR using 84759-4-RR (same clone as 84759-4-PBS)

    JAR cells were subjected to SDS PAGE followed by western blot with 84759-4-RR (CLIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using CLIC3 Recombinant antibody (84759-4-RR)

    WB analysis of HeLa using 84759-4-RR (same clone as 84759-4-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 84759-4-RR (CLIC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CLIC3 Recombinant antibody (84759-4-RR)

    IF Staining of A431 using 84759-4-RR (same clone as 84759-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CLIC3 antibody (84759-4-RR, Clone: 242326H7 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84759-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84759-4-RR

    Affinity and Kinetic Characterization of 84759-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84759-4-RR against Human CLIC3 were performed. The affinity constant is 0.634 nM.

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CLIC3 Recombinant antibody, PBS Only

CLIC3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 84759-4-PBS
Clone No.242326H7

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

242326H7

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84759-4-PBS targets CLIC3 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen CLIC3 fusion protein Ag8757 相同性解析による交差性が予測される生物種
Full Name chloride intracellular channel 3
Calculated molecular weight 236 aa, 26 kDa
Observed molecular weight 27-32 kDa
GenBank accession numberBC007012
Gene Symbol CLIC3
Gene ID (NCBI) 9022
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDO95833
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume (PMID: 9880541). CLIC3 (chloride intracellular channel protein 3) belongs to the chloride channel CLIC family which has seven members based on sequence homology to the p64 chloride channels of bovine kidney microsomes. CLIC proteins lack the membrane-spanning domains characteristic of channel proteins and are largely intracellular. CLIC3 is predominantly localized in the nucleus and has a high expression level in placenta (PMID: 9880541; 17027078). It associates with the C-terminal of ERK7 and may participate in cellular growth control.